Journal: Current biology : CB

Article Title: Nuclear deformation causes DNA damage by increasing replication stress

doi: 10.1016/j.cub.2020.11.037

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-phospho-histone H2A.X (ser 139) Millipore Cat# 05–636-I anti-phospho-RPA32 (S33) Bethyl Laboratories Cat# A300–246A-M anti-lamin A/C (E-1) Santa Cruz Biotechnology Cat# sc-376248 anti-histone H3 (1B1B2) Abcam Cat# ab195277 Alexa Fluor 647; Donkey anti-rabbit Invitrogen Cat# A-31573 Alexa Fluor 488 Azide, Bis, 5-isomer Invitrogen Cat# A10266 Alexa Fluor 488; Donkey anti-mouse Invitrogen Cat# A-21202 IRDye® 680RD Donkey anti-Mouse IgG LI-COR Cat# 926–68072 Chemicals, Peptides, and Recombinant Proteins Phleomycin Cayman Chemical Cat# 11549; CAS# 11006–33–0 Fibronectin Millipore Cat# FC010 Hoechst 33422 Invitrogen Cat# H3570 Dulbecco’s Modified Eagle Medium ThermoFisher Cat# 11965–092 Fetal Bovine Serum VWR Cat# 89510–186 Penicillin Streptomycin ThermoFisher Cat# 15070–063 Blasticidine S Invivogen Cat# ant-bl-1 Puromycin Invivogen Cat# ant-pr-1; CAS# 58–58–2 PureFection transfection reagent SBI Cat# LV750A-1 Type I – rat tail collagen Corning Cat# 354236 Polybrene infection/transfection reagent Sigma-Aldrich Cat# TR-1003 RNase A Omega Bio-Tek Cat# SKU:AC118 cOmplete EDTA-free protease inhibitor cocktail Roche Cat# 11873580001 PhosSTOP Roche Cat# 4906845001 Hydroxyurea ACROS Organics Cat# AC151680250 CAS# 127–07-1 5-Ethynyl-2’-deoxyuridine (5-EdU) Jena Biosciences Cat# CLK-N001–100 N-acetyl-L-cysteine Cayman Chemical Cat# 20261; CAS# 616–91–1 Propidium Iodide Invitrogen Cat# P3566 Critical Commercial Assays NucView 488 Caspase-3 assay kit for live cells Biotium Cat# 30029-T Experimental Models: Cell Lines MDA-MB-231 ATCC Cat# ATCC HTB-26 BT-549 ATCC Cat# ATCC HTB-122 HT1080 DSMZ Braunschweig, Germany Cat# ACC 315 SV40 immortalized human fibroblasts Coriell Instiute Cat# GM00637 hTERT RPE-1 Smolka lab, Cornell University Cat# ATCC CRL-4000 Recombinant DNA mCherry-BP1–2-pLPC-Puro Addgene Plasmid #19835 pLenti6.2-IRES-G1-Orange-S-G2-M-Green-BlastiS Katarina Wolf, Radboud UMC N/A pHAGE-CMV-GFP-PCNA-BlastiS Nina Mommasaparat; Washington University, St. Louis N/A pCDH-CMV-NLS-copGFP-EF1-blastiS Addgene Plasmid #132772 pCDH-CMV-H2B-mScarlet-EF1-Puro This paper N/A Software and Algorithms FIJI-ImageJ NIH https://imagej.nih.gov/ij GraphPad Prism 8 GraphPad https://www.graphpad.com/scientific-software/prism/ Adobe Illustrator CC 2018 Adobe https://www.adobe.com/products/illustrator.html Imaris 9.5 BitPlane https://imaris.oxinst.com/ Open in a separate window KEY RESOURCES TABLE DNA damage incurred during confined migration can result from multiple mechanisms.

Techniques: Recombinant, Modification, Transfection, Infection, Protease Inhibitor, Caspase-3 Assay, Plasmid Preparation, Software